Product Introduction：

LipoMax is a liquid reagent with a concentration of 5mg/ml. Save at 4 degrees. Do not freeze.

product quality：

The LipoMax transfection reagent has undergone extensive testing in HEK293 and mouse trophoblast stem cells (TS). The LipoMax transfection reagent is free from microbial contamination.

Important reference

1. The ratio of DNA (µg) to LipoMax (µl) is suitable between 1:2 and 1:3. Taking one well of a 24 - well plate as an example, for transfecting 0.5 - 2.0×10⁵ cells, 0.5 - 1 µg of DNA can be mixed with 1 - 3 µl of LipoMax. For specific experiments, adjusting the DNA/LipoMax ratio can optimize the transfection efficiency.

2. To ensure the transfection efficiency, the cell density during transfection for most cells should reach 60 - 80%. However, the specific cell density should be determined based on the optimized conditions.

3. There is no need to use antibiotic - free culture medium during transfection.

4.Opti - MEM or other serum - free culture media, such as DMEM, can be used to dilute DNA and LipoMax. If other serum - free culture media are used for dilution, the conditions need to be optimized.